{"entities":{"Q6692665":{"pageid":14411645,"ns":120,"title":"Item:Q6692665","lastrevid":40422234,"modified":"2025-03-03T10:38:34Z","type":"item","id":"Q6692665","labels":{"en":{"language":"en","value":"Functional memory T cells are derived from exhausted clones and expanded by checkpoint blockade [scRNA/TCR-seq]"}},"descriptions":{"en":{"language":"en","value":"Dataset published at Zenodo repository."}},"aliases":{},"claims":{"P31":[{"mainsnak":{"snaktype":"value","property":"P31","hash":"dae155fd0809a7906855cd4fa50dd7d71bed552b","datavalue":{"value":{"entity-type":"item","numeric-id":56885,"id":"Q56885"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$95764CE4-C011-45DB-80BA-96C4EF231F19","rank":"normal"}],"P1459":[{"mainsnak":{"snaktype":"value","property":"P1459","hash":"30349a12fed8e937066e702377164c20c663b7b7","datavalue":{"value":"Seurat Objects froms scRNA/TCR-seq Data Associated with the manuscript: Functional memory T cells are derived from exhausted clones and expanded by checkpoint blockade This dataset is a supplement to our Github Repostory which contains raw data and R markdown files neccesary to generate all figures from our manuscript. Three (3) Seurat Objects are available on this page  scRNA/TCR-seq atlas: Analysis of scRNA/TCR-seq of GP33+ cells (+/- CD62L enrichment): Fig. 1,2 and Extended Data Fig. 1,3,4 Polyclonal Rechallenge: scRNA/TCR-seq from clonal tracing of CD8+ T cells from 100+ dpi of acuure and chronic infection during rechallenge: Fig. 4 and Extended Data Fig. 6,7 scRNA/TCR-seq aPDL1: Analysis of scRNA/TCR-seq of GP33+ cells after aPDL1 Treatment: Fig. 5 and Extended Data Fig. 8  Raw single cell data are on NCBI GEO at accession numbers GSE285411 (scRNA/TCR-seq atlas), GSE285414 (scRNA/TCR-seq post-rechallenge), and GSE285412 (scRNA/TCR-seq PD-L1). Manuscript Abstract: Immune checkpoint blockade can facilitate tumor clearance by T cells, resulting in long term patient survival. However, the capacity of exhausted CD8+ T cells (Tex), present during chronic antigen exposure, to form memory after antigen clearance remains unclear. Here, we performed longitudinal single cell RNA/T cell receptor sequencing and ATAC-sequencing on antigen-specific T cells after the peripheral clearance of chronic lymphocytic choriomeningitis virus infection. These data revealed the formation of a robust population of memory CD8+ T cells that transcriptionally, epigenetically, and functionally resemble central memory T cells (Tcm) that form after clearance of acute infection. To lineage trace the origin and memory recall response of Tex-derived memory clones, we utilized T cell receptor sequencing over the course of primary infection and rechallenge. We show that chronic Tcm are a clonally distinct lineage of Tex derived from progenitor exhausted cells, persist long-term in the absence of antigen, and undergo rapid clonal expansion during rechallenge. Finally, we demonstrate that PD-L1 immune checkpoint blockade selectively expands clones which form Tcm after clearance. Together, these data support the concept that chronically stimulated T cells form bona fide functional memory T cells through an analogous differentiation pathway to acutely stimulated T cells, which may have significant implications for enhancing immune memory to cancer through checkpoint blockade and vaccination.","type":"string"},"datatype":"string"},"type":"statement","id":"Q6692665$D5E22B17-DBB2-4377-9B30-207B01B62999","rank":"normal"}],"P28":[{"mainsnak":{"snaktype":"value","property":"P28","hash":"e2b10fff8c3252cc1662c09d69f1ec68581b268e","datavalue":{"value":{"time":"+2025-02-04T00:00:00Z","timezone":0,"before":0,"after":0,"precision":11,"calendarmodel":"http://www.wikidata.org/entity/Q1985727"},"type":"time"},"datatype":"time"},"type":"statement","id":"Q6692665$F810DEDA-5C89-4DFC-9228-43B8FEE30473","rank":"normal"}],"P16":[{"mainsnak":{"snaktype":"value","property":"P16","hash":"995a87a916be6b776e63608a65f491895173ed42","datavalue":{"value":{"entity-type":"item","numeric-id":6692662,"id":"Q6692662"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$8765938E-8FA5-4A7B-A4DD-2305EBDF67CD","rank":"normal"},{"mainsnak":{"snaktype":"value","property":"P16","hash":"42e345091393645e01e9c2a2b3b53e4c881f45cf","datavalue":{"value":{"entity-type":"item","numeric-id":6692663,"id":"Q6692663"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$E630A303-E48F-448F-A7B1-4ADFC88C3F29","rank":"normal"},{"mainsnak":{"snaktype":"value","property":"P16","hash":"917fb83f0099464ffcebe61fdf93d97ada9dde10","datavalue":{"value":{"entity-type":"item","numeric-id":137992,"id":"Q137992"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$7990ED29-DEDB-4BE5-A96D-7325C47811A7","rank":"normal"}],"P227":[{"mainsnak":{"snaktype":"value","property":"P227","hash":"1a088470c46d32bdf6a98f51e52cfaa8b3ff1f5b","datavalue":{"value":"14803877","type":"string"},"datatype":"external-id"},"type":"statement","id":"Q6692665$CF869CDE-692B-4042-BF81-9BD429D53EDA","rank":"normal"}],"P27":[{"mainsnak":{"snaktype":"value","property":"P27","hash":"9d30be7d3c91cbcfc138dbb6e405e6ce158ffe9e","datavalue":{"value":"10.5281/zenodo.14803877","type":"string"},"datatype":"external-id"},"type":"statement","id":"Q6692665$BCEE89B2-1238-46F0-B519-3DBA10C4AE5F","rank":"normal"}],"P163":[{"mainsnak":{"snaktype":"value","property":"P163","hash":"45fcd4163b5f33e6e8c784f5522d7246c0a1a61e","datavalue":{"value":{"entity-type":"item","numeric-id":57056,"id":"Q57056"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$EDD9D186-7C66-45FA-9391-7D86A89CF340","rank":"normal"}],"P1460":[{"mainsnak":{"snaktype":"value","property":"P1460","hash":"d1e8073b72a070520efd3d14d4b3d2d3d03859e2","datavalue":{"value":{"entity-type":"item","numeric-id":5984635,"id":"Q5984635"},"type":"wikibase-entityid"},"datatype":"wikibase-item"},"type":"statement","id":"Q6692665$0B457089-26B9-4A42-A796-3EC7F2EBF113","rank":"normal"}]},"sitelinks":{"mardi":{"site":"mardi","title":"Dataset:6692665","badges":[]}}}}}