Spatial transcriptomics defines injury specific microenvironments and cellular interactions in kidney regeneration and disease
DOI10.5281/zenodo.12737086Zenodo12737086MaRDI QIDQ6674965FDOQ6674965
Dataset published at Zenodo repository.
Michal Polonsky, Andrew McMahon, Shiwei Zheng, long cai, Jina Yun, Guo-Cheng Yuan, Katsuya Colón, Kari Koppitch, Louisa Gerhardt, Matt Thomson, Barbara Wold, Henry Amrhein
Publication date: 13 July 2024
Kidney injury disrupts the intricate renal architecture and triggers limited regeneration, and injury-invoked inflammation and fibrosis. Deciphering molecular pathways and cellular interactions driving these processes is challenging due to the complex renal architecture. Here, we apply single cell spatial transcriptomics to examine ischemia-reperfusion injury in the mouse kidney. Spatial transcriptomics reveals injury-specific and spatially-dependent gene expression patterns in distinct cellular microenvironments within the kidney and predicts Clcf1-Crfl1 in a molecular interplay between persistently injured proximal tubule cells and neighboring fibroblasts. Immune cell types play a critical role in organ repair. Spatial analysis reveals cellular microenvironments resembling early tertiary lymphoid structures and identifies associated molecular pathways. Collectively, this study supports a focus on molecular interactions in cellular microenvironments to enhance understanding of injury, repair and disease.
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