Single-cell transcriptomic analysis of B cells reveals new insights into atypical memory B cells in COVID-19
DOI10.5281/zenodo.12519679Zenodo12519679MaRDI QIDQ6683227FDOQ6683227
Dataset published at Zenodo repository.
Adriana Karina Chavez-Rueda, Melissa García-Vega, Edgar Alonso Melgoza González, Olivia Valenzuela, Jesús Hernández, Diana Hinojosa, Veronica Mata-Haro, Miguel Hernández-Oñate, Mara Llamas-Covarrubias, Monica Resendiz, Martin Loza, Kenta Nakai
Publication date: 2 July 2024
Copyright license: Creative Commons Attribution 4.0 International
Here, we performed single-cell RNA sequencing of S1 and RBD protein-specific B cells from convalescent COVID-19 patients with different clinical manifestations. This study aimed to evaluate the role and developmental pathway of atypical memory B cells in response to SARS-CoV-2 infection. The results revealed a proinflammatory signature across B cell subsets associated with disease severity, as evidenced by the upregulation of genes such as GADD45B, MAP3K8, and NFKBIA in critical and severe individuals. Furthermore, the analysis of atypical memory B cells suggested a developmental pathway similar to that of conventional memory B cells through germinal centers, as indicated by the expression of several genes involved in germinal center processes, including CXCR4, CXCR5, BCL2, and MYC. Additionally, the upregulation of genes characteristic of the immune response in COVID-19, such as ZFP36 and DUSP1, suggested that the differentiation and activation of atypical memory B cells may be influenced by exposure to SARS-CoV-2 and that these genes may contribute to the immune response for COVID-19 recovery. Our study contributes to a better understanding of atypical memory B cells in COVID-19 and the role of other B cell subsets across different clinical manifestations.
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