Data for: Low protease activity in B cell follicles promotes retention of intact antigens after immunization
DOI10.5281/zenodo.7737955Zenodo7737955MaRDI QIDQ6683801FDOQ6683801
Dataset published at Zenodo repository.
William R. Schief, Murillo Silva, J. Christopher Love, Aereas Aung, Maurice Bukenya, Wuhbet Abraham, Laura Maiorino, Josetta Adams, Yiming Zhang, Heya Lee, Justin R. Gregory, Tanaka Remba, Christopher A. Cottrell, Phillip Huyett, Nir Hacohen, Ang Cui, Douglas S. Kwon, Leah M. Froehle, Duncan M. Morgan, Jesse D. Kirkpatrick, Shuhao Xiao, Darrell J. Irvine, Sangeeta N. Bhatia, Heikyung Suh, Ava P. Amini, Parastoo Amlashi
Publication date: 15 March 2023
The structural integrity of vaccine antigens is critical to the generation of protective antibody responses, but the impact of protease activity on vaccination in vivo is poorly understood. We characterized protease activity in lymph nodes and found that antigens were rapidly degraded in the subcapsular sinus, paracortex, and interfollicular regions, whereas low protease activity and antigen degradation rates were detected in the vicinity of follicular dendritic cells (FDCs). Correlated with these findings, immunization regimens designed to target antigen to FDCs led to germinal centers dominantly targeting intact antigen, whereas traditional immunizations led to much weaker responses that equally targeted the intact immunogen and antigen breakdown products. Thus, spatially compartmentalized antigen proteolysis affects humoral immunity and can be exploited.
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