RUCova: Removal of Unwanted Covariance in mass cytometry data
DOI10.5281/zenodo.10913464Zenodo10913464MaRDI QIDQ6690186FDOQ6690186
Dataset published at Zenodo repository.
Rosario Astaburuaga-García, Thomas Sell, Samet Mutlu, Anja Sieber, Nils Blüthgen, Kirsten Lauber
Publication date: 4 April 2024
Copyright license: Creative Commons Attribution 4.0 International
Mass cytometry data, cell area measurement data, and R Markdown reports to reproduce the figures of our publication and understand the application of RUCova. Raw data were saved post de-convolution and spillover-compensation. Gates for singlets and non-dead cells (low_Pt) are included as logical columns and should be applied prior to usage. Mass cytometry data sets: data1_HNSCC.csv - Data from 10 Head-and-neck squamous cell carcinoma (HNSCC) cell lines in control condition and 48 h after irradiation with 10 Gy. marker_category_HNSCC.csv - table for each marker in the mass cytometry panel and category. data2_FACS.csv - Data from Cal33 cell line under different stimuli and FACS-sorted by size (small and large). size_gates.png - Image depicting the FACS sorting. data3_ru.csv - Data from Cal33 cell line with Ruthenium staining (Rapsomaniki et. al. 2018, doi:10.1038/s41467-018-03005-5). data_ruthenium_rapsomaniki.csv (data from Rapsomaniki et. al. 2018) Cell area measurement data: cell_area_HNSCC.csv - Measurements of the cell area [m^2] via microscopy images for the 10 HNSCC in control condition and after irradiation with 10 Gy. R Markdown reports: analysis.Rmd analysis.html
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