Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis - Microscopy Data

DOI10.5281/zenodo.7767198Zenodo7767198MaRDI QIDQ6694289FDOQ6694289

Dataset published at Zenodo repository.

Adriano Caliari, Martin M. Hanczyc, Masayuki Imai, Jian Xu, Tetsuya Yomo

Publication date: 4 April 2023

Copyright license: Creative Commons Attribution 4.0 International

Description

Microscopy dataset of multipoint-multichannel images of giant unilamellar vesicles (GUVs) suspensions analysed inQuantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis (under revision). Three folders concerning different sections of the work are included. preliminary analysis.zip contians the raw files and analysis scripts for recall computation and imaging setup optimization as described in the paper. Timelapse data was excluded due to file size restrictions (available upon request at the corresponding authors of the work).IFC comparison.zip contains raw files and analysis scripts used to optimize colocalization computation in lipid exchange and content exchange experiments. GUV fusion analysis contains raw files and analysis scripts for the quantification of lipid and content exchange upon sodium chloride-induced aggregation. Further details on the analysis are provided in the paper. The R scripts require files saved upon analysis of the raw files by the ImageJ macro CE_analysis_CPU.ijm included here. The R environment of the complete analysis are included in each folder to provide easier access to the elaborated data.

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