Comprehensive Multimodal Immune Response Dataset for LAIV Vaccination in Pediatric Cohorts
DOI10.5281/zenodo.14719593Zenodo14719593MaRDI QIDQ6694831FDOQ6694831
Dataset published at Zenodo repository.
Adriana Tomic, Thushan de Silva, Ivan Tomic
Publication date: 22 January 2025
Copyright license: Creative Commons Attribution 4.0 International
This dataset is part of the publication "Integrative Mapping of Pre-existing Immune Landscapes for Vaccine Response Prediction". It provides detailed immune profiling of 244 children from The Gambia who received the trivalent live attenuated influenza vaccine (LAIV, Nasovac-S) during the 20162017 and 20172018 influenza seasons. It integrates multi-dimensional immune parameters across baseline and post-vaccination timepoints (day 0, day 2, day 7, and day 21) to capture individual immunogenicity profiles. The dataset includes: Humoral immune responses: Haemagglutination inhibition (HAI) assays, influenza virus protein microarray (IVPM), antibody-dependent cellular cytotoxicity (ADCC) activity, and enzyme-linked immunosorbent assay (ELISA) data for IgA and IgG levels. These measurements assess responses to vaccine-matched and cross-reactive influenza strains, including H1N1, H3N2, and B/Victoria. Cellular immune responses: T-cell cytokine production (e.g., interferon-gamma (IFN-) and interleukin-2 (IL-2)) measured via intracellular cytokine staining (ICS) following stimulation with hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), and matrix (M) protein peptides. Viral shedding and microbiome analysis: Viral load quantification by reverse-transcription PCR (RT-PCR) for vaccine strains (H1N1, H3N2, and Flu B) and nasopharyngeal microbiome profiling focusing on Streptococcus pneumoniae density. Immunophenotyping: Flow cytometry data on innate and adaptive immune cell subsets, including myeloid dendritic cells (mDCs), plasmacytoid dendritic cells (pDCs), monocyte subsets (classical, intermediate, and non-classical), and T follicular helper (Tfh) cells. Transcriptomics: RNA-sequencing (RNA-seq) data from nasal and blood samples, providing transcriptomic profiles analyzed through gene set enrichment analysis (GSEA). This dataset offers a unique resource for studying LAIV-induced immune responses and developing predictive models for vaccine immunogenicity, with applications in precision-guided vaccination strategies.
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