Identification of a Pathway for Electron Uptake in Shewanella oneidensis
DOI10.5281/zenodo.5013687Zenodo5013687MaRDI QIDQ6698968FDOQ6698968
Dataset published at Zenodo repository.
Liat H. Kugelmass, Farshid Salimijazi, Isao Anzai, Leah Trutschel, Buz Barstow, Michael Baym, Annette Rowe, Joshua Sackett, Oluwakemi Adesina
Publication date: 22 June 2021
Copyright license: Creative Commons Attribution 4.0 International
Data for figures for Identification of a Pathway for ElectronUptake inShewanella oneidensis Abstract Extracellular electron transfer (EET) could enable electron uptake into microbial metabolism for the synthesis of complex, energy dense organic molecules from CO2and renewable electricity1-6. Theoretically EET could do this with an efficiency comparable to H2-oxidation7,8but without the need for avolatile intermediateand the problems it causes for scale up9. However, significant gaps remain in understanding the mechanism and genetics of electron uptake. For example, studies of electron uptake in electroactive microbes have shown a role for the Mtr EET complex in the electroactive microbeShewanella oneidensisMR-110-14, though there is substantial variation in the magnitude of effect deletion of these genes has depending on the terminal electron acceptor used. This speaks to the potential for novel and/or differentially utilized genes involved in electron uptake. To address this, we screened gene disruption mutants for 3,667 genes, representing 99% of all non-essential genes, from theS. oneidensiswhole genome knockout collection using a redox dye oxidation assay. Confirmation of electron uptake using electrochemical testing allowed us to identify five genes fromS.oneidensisthat are indispensable for electron uptake from a cathode. Knockout of each gene eliminates extracellular electron uptake, yet in four of the five cases produces no significant defect in electron donation to an anode. This result highlights both distinct electron uptake components and an electronic connection between aerobic and anaerobic electron transport chains that allow electrons from the reversible EET machinery to be coupled to different respiratory processes inS. oneidensis. Homologs to these genes across many different genera suggesting that electron uptake by EET coupled to respiration could be widespread. These gene discoveries provide a foundation for: studying this phenotype in exotic metal-oxidizing microbes, genetic optimization of electron uptake inS. oneidensis; and genetically engineering electron uptake into a highly tractable host likeE. colito complement recent advances in synthetic CO2fixation15.
This page was built for dataset: Identification of a Pathway for Electron Uptake in Shewanella oneidensis
