Stability of oral and faecal microbiome at room temperature: impact on diversity
DOI10.5281/zenodo.7684999Zenodo7684999MaRDI QIDQ6702982FDOQ6702982
Dataset published at Zenodo repository.
Esther Saus, Blanca Rius-Sansalvador, Toni Gabaldon, Olfat Khannous-Lleiffe, Elisabet Guinó, Victor Moreno, Mireia Obon-Santacana, David Bars-Cortina, Ainhoa Garcia- Serrano
Publication date: 28 February 2023
Copyright license: Creative Commons Attribution 4.0 International
When collecting oral and faecal samples for large epidemiological microbiome studies, optimal storage conditions such as immediate freezing, are not always feasible. It is fundamental to study the impact of temporary room temperature (RT) storage and shipping on the microbiome diversity obtained in different types of samples. We performed a pilot study aimed to validate the sampling protocol based on the viability of the 16S rRNA gene sequencing in microbiome samples. Faecal and oral samples from five participants were collected and preserved in different conditions: i) 70% ethanol; ii) in a FIT tube for stool samples; and iii) in a chlorhexidine solution for oral wash samples. Four aliquots were prepared per sample, which were stored at RT, and frozen at days 0, 5, 10 and 15, respectively.
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