Datasets of "Differences in the stool metabolome between vegans and omnivores: analyzing the NIST stool reference material" publication

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Datasets of "Differences in the stool metabolome between vegans and omnivores: analyzing the NIST stool reference material" publication



DOI10.5281/zenodo.7977475Zenodo7977475MaRDI QIDQ6709877FDOQ6709877

Dataset published at Zenodo repository.

Zakery Tippins, Tong Shen, Oliver Fiehn, Bennett D Haffner, Raquel Cumeras, Luis M Valdiviez

Publication date: 27 May 2023

Copyright license: Creative Commons Attribution 4.0 International



To gain confidence in results of omic-data acquisitions, methods must be benchmarked by validated quality control materials. We here report data combining both untargeted and targeted metabolomics assays for the analysis of four new human fecal reference materials developed by the U.S. National Institute of Standards and Technologies (NIST) for metagenomics and metabolomics measurements. These reference grade test materials (RGTM) were established by NIST based on two different diets and two different samples treatments: homogenized fecal matter from subjects eating vegan diets, stored and submitted in either lyophilized (RGTM 10162) or aqueous form (RGTM 10171); secondly, homogenized fecal matter from subjects eating omnivore diets, stored and submitted in either lyophilized (RGTM 10172) or aqueous form (RGTM 10173). We used four untargeted metabolomics assays (lipidomics, primary metabolites, biogenic amines and polyphenols) and one targeted assay on bile acids.







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