snATAC-seq and snRNA-seq in the chromatin landscape of healthy and injured cell types in the human kidney

DOI10.5281/zenodo.8029990Zenodo8029990MaRDI QIDQ6717937FDOQ6717937

Dataset published at Zenodo repository.

Debora L. Gisch, Blue Lake, Michael T. Eadon, Sanjay Jain

Publication date: 12 June 2023

Description

Isolated nuclei were immediately processed using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (v1.0) kit, following the step-by-step protocol provided online [1].The RNA and ATAC libraries were separately sequenced on the NovaSeq 6000 system (Illumina) using the NovaSeq Control Software versions 1.7.0 and 1.7.5. Sample demultiplexing, barcode processing, gene expression quantification, and open chromatin peak quantification were performed using the Cell Ranger Arc software (version 2.0.0) with the GRCh38 (hg38) reference genome. Published in:DOI: 10.1038/s41467-023-44467-6

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