Data for: Optogenetic olfactory behavior depends on illumination characteristics

DOI10.5281/zenodo.2541462Zenodo2541462MaRDI QIDQ6720016FDOQ6720016

Dataset published at Zenodo repository.

Safwan B. Burhanudin, Tayfun Tumkaya, James Stewart, Adam Claridge-Chang

Publication date: 16 January 2019

Copyright license: Creative Commons Attribution 4.0 International

Description

Abstract Optogenetics has become an important tool to study behavior: it enables neuroscientists to infer causations by examining animal behavior after activating genetically circumscribed neurons with light. Light-induced activity is profoundly affected by illumination parameters used in experiments, such as intensity, duration, and frequency. How sensitive behavioral outcomes to light-dependent spike changes has not been extensively studied. Here, we investigated the hypothesis that light frequency would alter optogenetically induced behaviours. To test this, we activated olfactory receptor neurons (ORNs) in Drosophila by using either static- or pulsed-light stimuli. Static- and pulsed-light stimulations elicited distinct valence responses (attraction, aversion, neutral) to artificial activity in ORNs. Our results demonstrate the importance of light frequency for interpreting behavioral experiments accurately, and suggest that multiple light parameters should be tested before generalizing behavioral experiment results.

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