SAXS-based titrations of periplasmic binding proteins against known ligands

DOI10.5281/zenodo.3355877Zenodo3355877MaRDI QIDQ6767881FDOQ6767881

Dataset published at Zenodo repository.

Pochia Chen, Kathryn Perez, Janosch Hennig, Pawel Masiewicz

Publication date: 31 July 2019

Copyright license: Creative Commons Attribution 4.0 International

Description

This dataset contains the small-angle X-ray scattering intensities of a series of titrations between proteins HisBP, GlnBP, and DEBP against a number of amino acids. A total of five synchrotron beamline sessions are included to test for reproducibility across setups and total exposure time per sample: DESY P12 (4s) ESRF BM29 (5s) Australian Synchrotron SAXS/WAXS (20s) Diamond B21 (40s) The accompanying manuscripts can be found on bioRxiv MS 715193, entitled: Structure-based screening of binding affinities via small-angle X-ray scattering. It is currently under consideration with te Biophysical Journal.

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