diffwrap
Functions for differential expression analysis of read counts from messenger RNA (mRNA) sequencing (RNA-Seq) data or micro RNA (miRNA) expression values generated by the Comprehensive Analysis Pipeline for microRNA Sequencing (CAP-miRSeq) 'expression_reports.sh' script. The workflow follows the 'edgeR'-'limma' expression data analysis pipeline providing options for different approaches, such as "pure" 'edgeR', voom or paired samples. The functions in the package generate text files with differential expression lists, optionally annotated with information from 'biomart', expression summary plots as well as several quality control (QC) plots. The main function, diffExpr(), is a convenience wrapper performing all steps automatically based on sensible defaults. Methods are described in Robinson, McCarthy and Smyth (2010) <doi:10.1093/bioinformatics/btp616>, Ritchie et al. (2015) <doi:10.1093/nar/gkv007>, Law et al. (2014) <doi:10.1186/gb-2014-15-2-r29> and Sun et al. (2014) <doi:10.1186/1471-2164-15-423>.
- CAP-miRSeq: a comprehensive analysis pipeline for microRNA sequencing data
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
- limma powers differential expression analyses for RNA-sequencing and microarray studies
- voom: precision weights unlock linear model analysis tools for RNA-seq read counts
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