Modeling and optimization of genetic screens via RNA interference and FACS

DOI10.1017/S0269964814000254MaRDI QIDQ5358030zbMATH OpenOpenAlexFDO

Authors Y. Goldberg, Yuval Nov

Publication date 19 September 2017

Published in Probability in the Engineering and Informational Sciences (Search for Journal in Brave)

Full work available at URL https://arxiv.org/abs/1402.0172

zbMATH Keywords

fluorescence-activated cell sortermultinomial modelRNA interference

Mathematics Subject Classification ID

Applications of statistics to biology and medical sciences; meta analysis (62P10) Biochemistry, molecular biology (92C40) Genetics and epigenetics (92D10)

Abstract: We study mathematically a method for discovering which gene is related to a cell phenotype of interest. The method is based on RNA interference -- a molecular process for gene deactivation -- and on coupling the phenotype with fluorescence in a FACS machine. A small number of candidate genes are thus isolated, and then tested individually. We model probabilistically this process, prove a limit theorem for its outcome, and derive operational guidelines for maximizing the probability of successful gene discovery.

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Cites work

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• DNA Sequencing by Hybridization via Genetic Search
• Immortal branching Markov processes: Averaging properties and PCR applications.
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Cited in

(3)

• Systematic discovery of the grammar of translational inhibition by RNA hairpins
• A Bayesian measurement error model for two-channel cell-based RNAi data with replicates
• Sustainability of RNA-interference in rule based modelling

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