Automated and Robust Quantification of Colocalization in Dual-Color Fluorescence Microscopy: A Nonparametric Statistical Approach

DOI10.1109/TIP.2017.2763821MaRDI QIDQ4617023zbMATH OpenDBLPWikidataFDO

Authors Shulei Wang, Ellen T. Arena, Kevin W. Eliceiri, Ming Yuan

Publication date 6 February 2019

Published in IEEE Transactions on Image Processing (Search for Journal in Brave)

Full work available at URL https://arxiv.org/abs/1710.00965

Mathematics Subject Classification ID

Image processing (compression, reconstruction, etc.) in information and communication theory (94A08)

Abstract: Colocalization is a powerful tool to study the interactions between fluorescently labeled molecules in biological fluorescence microscopy. However, existing techniques for colocalization analysis have not undergone continued development especially in regards to robust statistical support. In this paper, we examine two of the most popular quantification techniques for colocalization and argue that they could be improved upon using ideas from nonparametric statistics and scan statistics. In particular, we propose a new colocalization metric that is robust, easily implementable, and optimal in a rigorous statistical testing framework. Application to several benchmark datasets, as well as biological examples, further demonstrates the usefulness of the proposed technique.

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