SPAD-FCS data of freely diffusing Alexa 488 AB
DOI10.5281/zenodo.4161418Zenodo4161418MaRDI QIDQ6708391FDOQ6708391
Dataset published at Zenodo repository.
Mauro Buttafava, Alberto Tosi, Sami V. Koho, Marco Castello, Eli Slenders, Luca Lanzano', Federica Villa, Giuseppe Vicidomini
Publication date: 30 October 2020
Copyright license: Creative Commons Attribution 4.0 International
Confocal laser-scanning microscopy based fluorescence fluctuation spectroscopy (FFS) data set of freely diffusing Alexa 488 linked to an antibody. Excitation at 485 nm, 100x/1.4 Leica objective, detection with a 5x5 pixel SPAD array detector. The file 05_Alexa488AB_20ugmL_P60percP_SPAD.7z contains a binary data file with the raw data of a 200 s FFS measurement. For each time bin of 500 ns, the fluorescence photon counts in each of the 25 pixels are stored. The text file 05_Alexa488AB_20ugmL_P60percP_SPAD_info.txt contains the metadata. The file 05_Alexa488AB_20ugmL_P60percP_SPAD_xcorrs.7z contains all 625 cross-correlations between I_1(t) and I_2(t), with I_1 and I_2 the intensity traces of any of the 25 pixels.
This page was built for dataset: SPAD-FCS data of freely diffusing Alexa 488 AB
