renz

R-Enzymology

Last update: 27 November 2023

Copyright license: GNU General Public License, version 3.0, GNU General Public License, version 2.0

Software version identifier: 0.1.1, 0.2.1

Description

Contains utilities for the analysis of Michaelian kinetic data. Beside the classical linearization methods (Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf and Eisenthal-Cornish-Bowden), features include the ability to carry out weighted regression analysis that, in most cases, substantially improves the estimation of kinetic parameters (Aledo (2021) <doi:10.1002/bmb.21522>). To avoid data transformation and the potentialbiases introduced by them, the package also offers functions to directly fitting data to the Michaelis-Menten equation, either using ([S], v) or (time, [S]) data. Utilities to simulate substrate progress-curves (making use of the Lambert W function) are also provided. The package is accompanied of vignettes that aim to orientatethe user in the choice of the most suitable method to estimate the kinetic parameter of an Michaelian enzyme.

Cites work

Enzyme kinetic parameters estimation: A tricky task?

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