flippant
CRANflippantMaRDI QIDQ130478FDOQ130478
Dithionite Scramblase Assay Analysis
Johannes Graumann, Richard J. Cotton
Last update: 27 November 2023
Copyright license: GNU General Public License
Software version identifier: 1.5.3, 1.0.2, 1.1.0, 1.5.2, 1.5.4
The lipid scrambling activity of protein extracts and purified scramblases is often determined using a fluorescence-based assay involving many manual steps. flippant offers an integrated solution for the analysis and publication-grade graphical presentation of dithionite scramblase assays, as well as a platform for review, dissemination and extension of the strategies it employs. The package's name derives from a play on the fact that lipid scrambling is also sometimes referred to as 'flipping'. The package is originally published as Cotton, R.J., Ploier, B., Goren, M.A., Menon, A.K., and Graumann, J. (2017). "flippant–An R package for the automated analysis of fluorescence-based scramblase assays." BMC Bioinformatics 18, 146. <doi:10.1186/s12859-017-1542-y>.
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