Nonlinear parameter estimation (uni uni reaction without product - Michaelis Menten model - steady state assumption)

nonlinear fit to determine the limiting reaction rate and michaelis constant.

computational task	nonlinear task

Available identifiers

Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

substrate concentration	input
initial reaction rate	input
Michaelis Menten equation (uni uni reaction without product - steady state assumption)	$v_{0} = \frac{V_{m a x, f} c_{S}}{K_{S} + c_{S}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Item	Property
nonlinear parameter estimation of enzyme kinetics	specialized by
uni uni reaction (Michaelis Menten model without product - steady state assumption)	used by

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