Linear parameter estimation (uni uni reaction without product - Hanes Woolf model - irreversibility assumption)

linear fit to determine the limiting reaction rate and michaelis constant.

computational task	linear task

Available identifiers

limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

initial reaction rate	input
substrate concentration	input
Michaelis constant substrate (uni uni reaction - irreversibility assumption)	$K_{S} \equiv \frac{k_{2}}{k_{1}}$	output
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - irreversibility assumption)

Hanes Woolf equation (uni uni reaction without product - irreversibility assumption)	$\frac{c_{S}}{v_{0}} = \frac{1}{V_{m a x, f}} c_{S} + \frac{K_{S}}{V_{m a x, f}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - irreversibility assumption)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Item	Property
linear parameter estimation of enzyme kinetics	specialized by
nonlinear parameter estimation (uni uni reaction without product - Michaelis Menten model - irreversibility assumption)	linearized by
uni uni reaction (Hanes Woolf model without product - irreversibility assumption)	used by

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