Linear parameter estimation (uni uni reaction without product - Lineweaver Burk model - steady state assumption)

linear fit to determine the limiting reaction rate and Michaelis constant.

computational task	linear task

Available identifiers

initial reaction rate	input
substrate concentration	input
Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

Lineweaver Burk equation (uni uni reaction without product - steady state assumption)	$\frac{1}{v_{0}} = \frac{1}{V_{m a x, f}} + \frac{K_{S}}{V_{m a x, f}} \frac{1}{c_{S}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Item	Property
linear parameter estimation of enzyme kinetics	specialized by
nonlinear parameter estimation (uni uni reaction without product - Michaelis Menten model - steady state assumption)	linearized by
uni uni reaction (Lineweaver Burk model without product - steady state assumption)	used by

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