Nonlinear parameter estimation (uni uni reaction without product and mixed complete inhibition - Michaelis Menten model - steady state assumption)

nonlinear fit to determine the limiting reaction rate, michaelis constant and inhibition constants.

computational task	nonlinear task

Available identifiers

MaRDI QIDQ6684590MaRDI QIDQ6684590FDOQ6684590

Contained entities

Michaelis Menten equation (uni uni reaction without product and mixed complete inhibition - steady state assumption)	$v_{0} = \frac{V_{m a x, f} c_{S}}{K_{S} (1 + \frac{c_{I}}{K_{i c}}) + (1 + \frac{c_{I}}{K_{i u}}) c_{S}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{I}$ represents inhibitor concentration
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

competitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i c} \equiv \frac{k_{- 3}}{k_{3}}$	output
	$k_{3}$ represents reaction rate constant
	$k_{- 3}$ represents reaction rate constant
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)

inhibitor concentration	input
limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

uncompetitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i u} \equiv \frac{k_{- 4}}{k_{4}}$	output
	$k_{4}$ represents reaction rate constant
	$k_{- 4}$ represents reaction rate constant
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)

initial reaction rate	input
Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

substrate concentration	input

Further links

Item	Property
nonlinear parameter estimation of enzyme kinetics	specialized by
uni uni reaction mixed complete inhibition (Michaelis Menten model without product - steady state assumption)	used by

Edit nonlinear parameter estimation (uni uni reaction without product and mixed complete inhibition - Michaelis Menten model - steady state assumption) item

This page was built for task: nonlinear parameter estimation (uni uni reaction without product and mixed complete inhibition - Michaelis Menten model - steady state assumption)