linear parameter estimation (uni uni reaction without product and mixed complete inhibition - Eadie Hofstee model - steady state assumption)

Community: MathModDB

computational task	linear task

Available identifiers

MaRDI QIDQ6684591

linear fit to determine the limiting reaction rate, michaelis constant and inhibition constants, named after Barend Hendrik Jan Hofstee, George Sharpe Eadie

List of contained entities

Eadie Hofstee equation (uni uni reaction without product and mixed complete inhibition - steady state assumption)	$v_{0} = \frac{V_{m a x, f}}{1 + \frac{c_{I}}{K_{i u}}} - \frac{K_{m} (1 + \frac{c_{I}}{K_{i c}})}{1 + \frac{c_{I}}{K_{i u}}} \frac{v_{0}}{c_{S}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{I}$ represents inhibitor concentration
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

competitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i c} \equiv \frac{k_{- 3}}{k_{3}}$	output
	$k_{3}$ represents reaction rate constant
	$k_{- 3}$ represents reaction rate constant
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)

inhibitor concentration
initial reaction rate
limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

substrate concentration
uncompetitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i u} \equiv \frac{k_{- 4}}{k_{4}}$	output
	$k_{4}$ represents reaction rate constant
	$k_{- 4}$ represents reaction rate constant
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)

Further items linking to linear parameter estimation (uni uni reaction without product and mixed complete inhibition - Eadie Hofstee model - steady state assumption)

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nonlinear parameter estimation (uni uni reaction without product and mixed complete inhibition - Michaelis Menten model - steady state assumption)	linearized by
uni uni reaction mixed complete inhibition (Eadie Hofstee model without product - steady state assumption)	used by

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