Linear parameter estimation (uni uni reaction without product and mixed complete inhibition - Hanes Woolf model - steady state assumption)

linear fit to determine the limiting reaction rate, michaelis constant and inhibition constants.

computational task	linear task

Available identifiers

MaRDI QIDQ6684592MaRDI QIDQ6684592FDOQ6684592

Contained entities

uncompetitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i u} \equiv \frac{k_{- 4}}{k_{4}}$	output
	$k_{4}$ represents reaction rate constant
	$k_{- 4}$ represents reaction rate constant
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)

inhibitor concentration	input
limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

substrate concentration	input
competitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i c} \equiv \frac{k_{- 3}}{k_{3}}$	output
	$k_{3}$ represents reaction rate constant
	$k_{- 3}$ represents reaction rate constant
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)

initial reaction rate	input
Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

Hanes Woolf equation (uni uni reaction without product and mixed complete inhibition - steady state assumption)	$\frac{c_{S}}{v_{0}} = \frac{c_{S} (1 + \frac{c_{I}}{K_{i u}})}{V_{m a x, f}} + \frac{K_{m} (1 + \frac{c_{I}}{K_{i c}})}{V_{m a x, f}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{I}$ represents inhibitor concentration
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Further links

Item	Property
linear parameter estimation of enzyme kinetics	specialized by
nonlinear parameter estimation (uni uni reaction without product and mixed complete inhibition - Michaelis Menten model - steady state assumption)	linearized by
uni uni reaction mixed complete inhibition (Hanes Woolf model without product - steady state assumption)	used by

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