nonlinear parameter estimation (uni uni reaction without product and non-competitive partial inhibition - Michaelis Menten model - steady state assumption)

Community: MathModDB

computational task	nonlinear task

Available identifiers

MaRDI QIDQ6684613

nonlinear fit to determine the limiting reaction rate, michaelis constant and inhibition constants, named after Leonor Michaelis, Maud Menten

List of contained entities

Michaelis Menten equation (uni uni reaction without product and non-competitive partial inhibition - steady state assumption)	$v_{0} = \frac{(V_{m a x, f} + \frac{V_{m a x, I, f} c_{I}}{K_{i u}}) c_{S}}{K_{S} (1 + \frac{c_{I}}{K_{i c}}) + (1 + \frac{c_{I}}{K_{i u}}) c_{S}}$
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)
	$V_{m a x, I, f}$ represents limiting reaction rate with inhibitor (uni uni reaction - forward)
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)
	$c_{I}$ represents inhibitor concentration
	$c_{S}$ represents substrate concentration
	$v_{0}$ represents initial reaction rate

Michaelis constant substrate (uni uni reaction - steady state assumption)	$K_{S} \equiv \frac{k_{- 1} + k_{2}}{k_{1}}$	output
	$k_{- 1}$ represents reaction rate constant
	$k_{1}$ represents reaction rate constant
	$k_{2}$ represents reaction rate constant
	$K_{S}$ represents Michaelis constant substrate (uni uni reaction - steady state assumption)

competitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i c} \equiv \frac{k_{- 3}}{k_{3}}$	output
	$k_{3}$ represents reaction rate constant
	$k_{- 3}$ represents reaction rate constant
	$K_{i c}$ represents competitive inhibition constant (uni uni reaction reversible inhibition)

inhibitor concentration
initial reaction rate
limiting reaction rate (uni uni reaction - forward)	$V_{m a x, f} \equiv k_{2} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{2}$ represents reaction rate constant
	$V_{m a x, f}$ represents limiting reaction rate (uni uni reaction - forward)

limiting reaction rate with inhibitor (uni uni reaction - forward)	$V_{m a x, I, f} \equiv k_{6} c_{E_{0}}$	output
	$c_{E_{0}}$ represents enzyme concentration
	$k_{6}$ represents reaction rate constant
	$V_{m a x, I, f}$ represents limiting reaction rate with inhibitor (uni uni reaction - forward)

substrate concentration
uncompetitive inhibition constant (uni uni reaction reversible inhibition)	$K_{i u} \equiv \frac{k_{- 4}}{k_{4}}$	output
	$k_{4}$ represents reaction rate constant
	$k_{- 4}$ represents reaction rate constant
	$K_{i u}$ represents uncompetitive inhibition constant (uni uni reaction reversible inhibition)

Further items linking to nonlinear parameter estimation (uni uni reaction without product and non-competitive partial inhibition - Michaelis Menten model - steady state assumption)

Item	Property
nonlinear parameter estimation of enzyme kinetics	specialized by
uni uni reaction non-competitive partial inhibition (Michaelis Menten model without product - steady state assumption)	used by

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