nonlinear parameter estimation (Hill enzyme kinetics model)

Community: MathModDB

computational task

nonlinear task

Available identifiers

MaRDI QIDQ6775836

nonlinear estimation of Hill enzyme kinetics parameters, named after Terrell L. Hill

Description

This task performs nonlinear parameter estimation for the Hill enzyme-kinetics model using initial reaction-rate data. It determines the maximal catalytic rate, the Hill kinetic constant, and the cooperativity exponent by fitting the nonlinear Hill velocity equation directly to substrate-concentration and reaction-velocity measurements. Nonlinear regression avoids biases that occur when kinetic data are linearized, producing more accurate parameter estimates for cooperative, allosteric, or multimeric enzymes. The resulting values characterize catalytic efficiency, apparent substrate affinity, and the degree of cooperativity in enzymatic turnover. This method is used in biochemistry and systems biology to analyze non-Michaelis–Menten reaction behavior.

List of contained entities

Hill coefficient
Hill limiting reaction rate
Hill velocity equation	$v_0=\frac{V_{max}{c}_S^{n_H}}{K_{0.5}^{n_H}+c_S^{n_H}}$
	$v_0$ represents initial reaction rate
	$n_H$ represents Hill coefficient
	$c_S$ represents substrate concentration
	$K_{0.5}$ represents half saturation constant (Hill kinetics)
	$V_{max}$ represents Hill limiting reaction rate
half saturation constant (Hill kinetics)
initial reaction rate
substrate concentration

Further items linking to nonlinear parameter estimation (Hill enzyme kinetics model)

Item	Property
Hill model (enzyme kinetics)	used by

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