A regularization procedure for estimating cell kinetic parameters from flow-cytometry data (Q1080794)

The problem of estimating kinetic parameters such as the DNA synthesis rate and the S-phase influx in a cell population monitored by flow cytometry is considered. The population can be in any growth state, assuming that there is no cell loss. The growth curve of the population and a sequence of DNA-content histograms are required data. Our approach, based on the use of continuity equations to model the population dynamics, utilizes a regularization procedure in order to filter the population-size data. Some properties of the procedure, together with an evaluation of its performance on simulated data, are presented.