Unsupervised particle sorting for high-resolution single-particle cryo-EM

DOI10.1088/1361-6420/AB5EC8zbMATH Open1470.92182arXiv1910.10051OpenAlexW3099491377MaRDI QIDQ5000566FDOQ5000566

Authors: Ye Zhou, Amit Moscovich, Tamir Bendory, Alberto Bartesaghi 

Publication date: 14 July 2021

Published in: Inverse Problems (Search for Journal in Brave)

Abstract: Single-particle cryo-Electron Microscopy (EM) has become a popular technique for determining the structure of challenging biomolecules that are inaccessible to other technologies. Recent advances in automation, both in data collection and data processing, have significantly lowered the barrier for non-expert users to successfully execute the structure determination workflow. Many critical data processing steps, however, still require expert user intervention in order to converge to the correct high-resolution structure. In particular, strategies to identify homogeneous populations of particles rely heavily on subjective criteria that are not always consistent or reproducible among different users. Here, we explore the use of unsupervised strategies for particle sorting that are compatible with the autonomous operation of the image processing pipeline. More specifically, we show that particles can be successfully sorted based on a simple statistical model for the distribution of scores assigned during refinement. This represents an important step towards the development of automated workflows for protein structure determination using single-particle cryo-EM.

Full work available at URL: https://arxiv.org/abs/1910.10051

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zbMATH Keywords

image analysiscryo-electron microscopysingle particle analysishigh-resolution microscopyunsupervised image sorting

Mathematics Subject Classification ID

Biomedical imaging and signal processing (92C55)

Cites Work

• Three-dimensional structure determination from common lines in cryo-EM by eigenvectors and semidefinite programming
• Angular synchronization by eigenvectors and semidefinite programming

Cited In (3)

• A max-cut approach to heterogeneity in cryo-electron microscopy
• An automatic particle picking method based on generative adversarial network
• Reconstruction from limited single-particle diffraction data via simultaneous determination of state, orientation intensity, and phase

Uses Software

• EMan
• DeepPicker
• CTFFIND
• RELION
• cisTEM
• IMAGIC
• cryoSPARC
• Leginon
• MAPPOS
• MotionCor2
• crYOLO

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