Correlation analysis of enzymatic reaction of a single protein molecule

DOI10.1214/12-AOAS541zbMATH Open1254.92034arXiv1209.6210OpenAlexW3105924497WikidataQ42687283 ScholiaQ42687283MaRDI QIDQ714353FDOQ714353

Authors: Chao Du, S. C. Kou 

Publication date: 21 October 2012

Published in: The Annals of Applied Statistics (Search for Journal in Brave)

Abstract: New advances in nano sciences open the door for scientists to study biological processes on a microscopic molecule-by-molecule basis. Recent single-molecule biophysical experiments on enzyme systems, in particular, reveal that enzyme molecules behave fundamentally differently from what classical model predicts. A stochastic network model was previously proposed to explain the experimental discovery. This paper conducts detailed theoretical and data analyses of the stochastic network model, focusing on the correlation structure of the successive reaction times of a single enzyme molecule. We investigate the correlation of experimental fluorescence intensity and the correlation of enzymatic reaction times, and examine the role of substrate concentration in enzymatic reactions. Our study shows that the stochastic network model is capable of explaining the experimental data in depth.

Full work available at URL: https://arxiv.org/abs/1209.6210

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zbMATH Keywords

single-molecule experimentcontinuous time Markov chainautocorrelationsfluorescence intensityMichaelis-Menten modelstochastic network modelturnover time

Mathematics Subject Classification ID

Applications of statistics to biology and medical sciences; meta analysis (62P10) Biochemistry, molecular biology (92C40) Systems biology, networks (92C42) Biophysics (92C05) Kinetics in biochemical problems (pharmacokinetics, enzyme kinetics, etc.) (92C45)

Cites Work

• Matrix Analysis
• Stochastic networks in nanoscale biophysics
• Title not available (Why is that?)
• Stochastic modeling in nanoscale biophysics: subdiffusion within proteins
• On the validity of the steady state assumption of enzyme kinetics
• Bayesian Analysis of Single-Molecule Experimental Data
• A selective view of stochastic inference and modeling problems in nanoscale biophysics

Cited In (6)

• The lack of long-range correlations is a necessary condition for a functional biologically active protein
• A selective view of stochastic inference and modeling problems in nanoscale biophysics
• Statistical methodology in single-molecule experiments
• Stochastic networks in nanoscale biophysics
• Stochastic modeling in nanoscale biophysics: subdiffusion within proteins
• Superiority of single covalent modification in specificity: from deterministic to stochastic viewpoint

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