Inverse problems of single molecule localization microscopy

DOI10.1007/978-3-030-57784-1_12MaRDI QIDQ3379573zbMATHOpenAlexFDO

Authors M. López-Martínez, Gwenael Mercier, Kamran Sadiq, Magdalena Schneider, John C. Schotland, Gerhard J. Schütz, Roger Telschow, Otmar Scherzer

Publication date 27 September 2021

Published in Time-dependent Problems in Imaging and Parameter Identification (Search for Journal in Brave)

Full work available at URL https://arxiv.org/abs/2002.01741

zbMATH Keywords

inverse problemsfluorescence microscopymolecule localization microscopy

Mathematics Subject Classification ID

Biochemistry, molecular biology (92C40) Biomedical imaging and signal processing (92C55) Biological applications of optics and electromagnetic theory (78A70) PDEs in connection with optics and electromagnetic theory (35Q60) Inverse problems for PDEs (35R30) Inverse problems (including inverse scattering) in optics and electromagnetic theory (78A46) Cell biology (92C37) Geometric optics (78A05)

Abstract: Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules, which after excitation with a strong laser may emit light. To achieve superresolution, signals of individual fluorophores are separated in time. In this paper we follow the physical and chemical literature and derive mathematical models describing the propagation of light emitted from dyes in single molecule localization microscopy experiments via Maxwell's equations. This forms the basis of formulating inverse problems related to single molecule localization microscopy. We also show that the current status of reconstruction methods is a simplification of more general inverse problems for Maxwell's equations as discussed here.

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Cites work

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Cited in

(7)

• Fluorescence image deconvolution microscopy via generative adversarial learning (FluoGAN)
• Research in Computational Molecular Biology
• A stochastic analysis of distance estimation approaches in single molecule microscopy: quantifying the resolution limits of photon-limited imaging systems
• A stochastic analysis of performance limits for optical microscopes
• Mathematical modeling for 2D light-sheet fluorescence microscopy image reconstruction
• Optimization problems in electron microscopy of single particles
• Reflection Methods for Inverse Problems with Applications to Protein Conformation Determination

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