A model for spheroid versus monolayer response of SK-N-SH neuroblastoma cells to treatment with 15-deoxy-PGJ₂

DOI10.1155/2016/3628124MaRDI QIDQ2011775zbMATH OpenOpenAlexDBLPWikidataFDO

Authors Ann Dunham, Paula X. Chen, Michelle Chen, Milan Huynh, Evan Rheingold, Olivia Prosper, Dorothy Wallace

Publication date 4 August 2017

Published in Computational \& Mathematical Methods in Medicine (Search for Journal in Brave)

Copyright license Creative Commons Attribution 4.0 International

Full work available at URL https://doi.org/10.1155/2016/3628124

zbMATH Keywords

15-deoxy-\(PGJ_2\)monolayer responseneuroblastoma cells treatmenttumor spheroid growth

Mathematics Subject Classification ID

Medical applications (general) (92C50)

Review text

Summary: Researchers have observed that response of tumor cells to treatment varies depending on whether the cells are grown in monolayer, as\textit{in vitro} spheroids or\textit{in vivo}. This study uses data from the literature on monolayer treatment of SK-N-SH neuroblastoma cells with 15-deoxy-\(PGJ_2\) and couples it with data on growth rates for untreated SK-N-SH neuroblastoma cells grown as multicellular spheroids. A linear model is constructed for untreated and treated monolayer data sets, which is tuned to growth, death, and cell cycle data for the monolayer case for both control and treatment with 15-deoxy-\(PGJ_2\). The monolayer model is extended to a five-dimensional nonlinear model of\textit{in vitro} tumor spheroid growth and treatment that includes compartments of the cell cycle (\(G_1, S, G_2/M\)) as well as quiescent (\(Q\)) and necrotic (\(N\)) cells. Monolayer treatment data for 15-deoxy-\(PGJ_2\) is used to derive a prediction of spheroid response under similar treatments. For short periods of treatment, spheroid response is less pronounced than monolayer response. The simulations suggest that the difference in response to treatment of monolayer versus spheroid cultures observed in laboratory studies is a natural consequence of tumor spheroid physiology rather than any special resistance to treatment.

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(7)

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